SpectraSplit® - Direct Multicolor Imaging

SpectraSplit® - Direct Multicolor Imaging of 7 or 10 Channels Without the Requirement of Spectral Unmixing or Post Processing

Visualizing four colors usually is not challenging with conventional bandpass filters. However, when you exceed four colors, the fluorochromes begin to bleed into each other’s channels.
The complete spectrum, from violet to far-red, can only be divided into a certain number of channels before spillover and non-specific signals arise. To achieve a clean signal, each channel must be narrow enough to prevent cross-talk – in other words, maintain specificity – while still capturing as much emission signal as possible.

We have optimized it for you with our patented SpectraSplit® 7, a 7-Channel filter set consisting of 4 bandpass filter sets, and SpectraSplit® 10, a 10-Channel filter set consisting of 5 bandpass filter sets.

Discover the Power of Seven Colors

Any high-throughput scanning method is enhanced by the use of more colors, as each staining cycle conveys more information. SpectraSplit® 7 is a patented set of bandpass filters that features carefully calculated and tested bandwidths. SpectraSplit^® 7 effectively separates seven common classes of fluorochromes without the need for spectral unmixing. The system is compatible with AlexaFluor^®dyes, as well as traditional FITC/Cy3/Cy5/Cy7 dyes and many varieties of Opal™ dyes. With its user-friendly design, simply choose one fluorochrome from each class to produce 7-color images free from channel spillover. The data generated requires no spectral unmixing or other types of post-processing. You can proceed directly to analysis with your preferred software. The power of these filter sets are described in a rapidly growing number of peer-reviewed publications.

Fluorochromes compatible with SpectraSplit^® 7

Channel	Traditional dyes	StreptaClick® dyes	ThermoFisher	Biotium	ThermoFisher	Atto-tec	Akoya	Ultivue	Sirigen/BD	Protein expression fluorophores
S-Split Blue (375)	DAPI	DAPI, Tyramide 405	Alexa Fluor 405	CF405	Dylight 405		DAPI	DAPI	BV421	Hoecht
S-Split Cyan (435)	CFP	Tyramide CF®430		CF430		Atto425	Opal p.480		BV480	cCFP
S-Split Green (490)	FITC	Tyramide 488, Color 488	Alexa Fluor 488	CF488	DyLight 488	Atto488	Opal 520	Ultivue, FITC		eGFP
S-Split Orange (545)	Cy3/TRITC	Tyramide 555, Color Atto 542	Alexa Fluor 546	CF555	DyLight 549	Atto542	Opal 570	Ultivue, Cy3		mOrange/ mRFP
S-Split Red (590)	Texas Red	Tyramide 594, Color 594	Alexa Fluor 594	CF594	DyLight 594	Atto590	Opal 620			mCherry/mRaspberry /mPlum
S-Split Far-red (650)	Cy5/Cy5.5	Tyramide 647, Color 647	Alexa Fluor 647	CF647/680	DyLight 649	Atto647/665	Opal 690	Ultivue, Cy5		miRFP703
S-Split Infra-red (740)	Cy7	Styramide iFlour 750	Alexa Fluor 750	CF750	DyLight 750	Atto740	Opal p.780	Ultivue, Cy7

*Cy3, Cy5, Cy7 are registered trademarks of GE Healthcare, Alexa Fluor and DyLight dyes are trademarks of Thermo Fisher Scientific, CF dyes are trademarks of Biotium, Atto dyes are trademarks of Atto-tec, Opal dyes are trademarks of Akoya Biosciences, Brilliant Violet dyes are trademarks of Sirigen/BD

Introducing the Unprecedented Imaging of Ten Colors Without Spectral Unmixing

SpectraSplit® 10 expands on our patented bandpass filter sets that features carefully calculated and tested bandwidths. SpectraSplit^® 10 effectively separates 9 common classes of fluorochromes from DAPI to Cy7 and and adds a channeld at 850 nm for emerging applications, all without the need for spectral unmixing. The system is compatible with AlexaFluor^®dyes, as well as traditional DAPI/CFP/FITC/Cy3/Texas Red/Cy5/Cy7 dyes and many varieties of Opal™ dyes. With its user-friendly design, simply choose one fluorochrome from each class to produce 10-color images free from channel spillover. The data generated requires no spectral unmixing or other types of post-processing. You can proceed directly to analysis with your preferred software.

Fluorochromes compatible with SpectraSplit^® 10

Common fluorophores compatible with SpectraSplit 10

Quality Filters

The filter sets are custom made by Chroma Technologies specifically for Kromnigon and manufactured to the high product standards of Chroma Technologies. SpectraSplit® 7 is a patented technology developed by Kromnigon, exclusive to our brand. These filter sets are not available under any other name.

SpectraSplit^® maximizes the capacity of any microscope

SpectraSplit^® 7 and SpectraSplit^® 10 immediately empowers your microscope with seven or ten independent color channels that each generates crystal clear images. SpectraSplit^® 7 and 10 are designed to work with both standard and scanning fluorescence microscopes. SpectraSplit^® 7 is compatible with various lighting sources from multiple manufacturers including CoolLED, Excelitas and Lumencor. SpectraSplit^® 10 was co-developed with CoolLED for the new pE-10 Illumination System, with both products launched simultaneously in April 2026.

SpectraSplit^® in the VS200 Research Slide Scanner from Evident

The SpectraSplit^® 7 is available as a seven-channel option on the VS200 Research Slide Scanner from Evident. In conjunction with a high-performance microscope, SpectraSplit^® 7 enhances your visual capabilities. For more information, please reach out to your Evident sales representative.

We’ve imaged over 2,500 highly-multiplexed slides with the SpectraSplit 7 filters in the past year and the data in every single sample set is crisp and clean. Our users do a large amount of RNAScope staining utilizing the Opal dye set and it’s been a game changer for us to have the SpectraSplit filters that are precisely matched to these dyes. The imaged samples have been a wide range of tissue sections and cultured cells and none of my other microscopes, both filter-based and with spectral detection, match the Opals as well as SpectraSplit 7.

Christina Baer,
Director, SCOPE Imaging Facility,
UMass Chan Medical School,
Worcester MA

Our company Offspring Biosciences supports project teams in the pharma industry with histology-based contract services. Kromnigons SpectraSplit filters have allowed us to significantly expand our capability to perform multiplexed immunofluorescence analyses with excellent separation between the fluorophores. We can highly recommend them.

Anders Dahlstrand,
Managing Director at Offspring
Bioscience

BiotinPure™ Antibody Biotinylation Kit

503 €

The BiotinPure™ Antibody Biotinylation Kit is a complete kit for easy biotinylation of antibodies at room temperature within 1 hour. The kit uses amine/ester chemistry to attach 2-3 biotins per antibody and includes a purification wash step that removes unreacted free biotin from the final biotinylated antibody solution. The antibodies to be biotinylated should be free from other proteins, such as BSA. The BiotinPure™ Antibody Biotinylation Kit is designed for use with StreptaClick® reagents. The biotinylated antibodies may also be used for other applications. Cat. no. 9000051-1 Order or request further information

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Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally - a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

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Mouse spleen stained with StreptaClick HRP 8-color, imaged with SpectraSplit 10 optical filter sets

SpectraSplit® 10 filter sets

SpectraSplit^® 10, developed from our patented SpectraSplit^® 7 filter sets, will revolutionize fluorescence microscopy as 10 channels can be directly imaged without spectral unmixing. The channels are distributed over five filter sets and separate the most commonly used fluorochromes in immunofluorescence, in addition to channels anticipated to gain popularity in the near future (see table below). Tissues and other sample types can be immunostained with up to 10 fluorochromes, visualized without bleedthrough. No additional software and no computational spectral unmixing are required. SpectraSplit^® 10 can easily be installed in both standard and scanning fluorescence microscopes. Request a quote or information

SpectraSplit® 7

SpectraSplit^® 7 are patented filter sets that empower fluorescence microscopes with seven color channels. The channels are distributed over four filter sets and specifically separate the most commonly used fluorochromes in immunofluorescence (see table below). Hence, you can immunostain tissues with up to seven fluorochromes and still visualize each fluorochrome in the sample with SpectraSplit^® 7 without bleedthrough. No additional software and no computational spectral unmixing are required. SpectraSplit^® 7 can easily be installed in both standard and scanning fluorescence microscopes, and can be run with different popular light sources. See the description below for more information. Cat no. 7001991-1 Request a quote or information

Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. We can also charge in $USD. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).

SpectraSplit® - Direct Multicolor Imaging