The StreptaClick® Technology
    - multiplex with biotinylated antibodies

    The patented StreptaClick® technology provides monovalent binding of streptavidin to biotinylated antibodies. StreptaClick® is a modified form of tetrameric streptavidin with conserved high binding affinity for biotin. Its monovalent binding property prevents antibody aggregation, which allows antibody pre-labeling in solution and multiplexing. StreptaClick® can be linked to any biotinylated antibody in a 5-minute mixing reaction, in any volume and without washing steps. Our product portfolio includes StreptaClick® labelled with fluorochromes, the enzyme horseradish peroxidase (HRP), oligonucleotides, and reactive groups for click chemistry.

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    SpectraSplit® 7 – Hepta-band optical filter sets for microscopes and scanners

    Specific separation of 7 fluorescent colors in a multi-stained tissue section has never been easier. The patented SpectraSplit® 7 filter sets generate clean, sharp images without the need for spectral unmixing, making it suitable for any downstream application. Simply select among the most popular fluorochromes for each color channel and image without bleed-through.

    SpectraSplit® 7 was initially developed in 2014. Over the years the hepta-band filter sets have been fine-tuned based on customer feedback to not only fit a wide range of fluorochromes, but also different staining techniques, including Tyramide Signal Amplification and DNA-based multiplexing. SpectraSplit® 7 is available in scanners from multiple manufacturers including Evident (VS200), TissueGnostics (TissueFAXS CHROMA), and Nikon (Ti2).

    SpectraSplit® 7 is compatible with light sources from CoolLED (pE-800 SB), Lumencor (SPECTRA X light engine) and Excelitas (NOVEM-S).

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    Heat-free multiplex TSA enabled by StreptaClick® HRP and Kromnigon's unique HRP block buffer

    Kromnigon’s StreptaClick® HRP multiplex IHC kits addresses the most common limitations of traditional multiplex tyramide signal amplification by removing the need for secondary antibodies and eliminating heat-based antibody stripping. HRP is directly linked to the primary antibody using StreptaClick®, and after each staining cycle the enzyme is specifically inactivated with a proprietary HRP blocking buffer before the next round begins.

    No heat is used to strip off antibodies between staining cycles, preserving tissue structure and epitope integrity. This allows the simultaneous analysis of multiple protein biomarkers in frozen and FFPE samples, and reduces the time to optimize antibody panels considerably.

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    Introducing StreptaClick® Precision

    A novel version of StreptaClick® developed for site-specific conjugation via click chemistry and enhanced monovalent binding to biotinylated proteins.

    StreptaClick® Precision has a unique design with maximum separation of the single reactive site for conjugation (azide/DBCO) and the site for biotin binding. This makes StreptaClick® Precision ideal for attachment of bulky molecules such as DNA oligos and LNPs.

    StreptaClick® Precision is the ultimate choice for method development requiring both high precision and flexibility. Possible applications include DNA origami, custom bead or LNP coating, antigen labeling, and highly multiplexed immunoassays.

    Perfect for oligonucleotides and lipid nanoparticles

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    Explore our product families

    SpectraSplit® 7

    SpectraSplit® 7 are patented filter sets that empower fluorescence microscopes with seven color channels.  The channels are distributed over four filter sets and specifically separate the most commonly used fluorochromes in immunofluorescence (see table below). Hence, you can immunostain tissues with up to seven fluorochromes and still visualize each fluorochrome in the sample with SpectraSplit® 7 without bleedthrough. No additional software and no computational spectral unmixing are required. SpectraSplit® 7 can easily be installed in both standard and scanning fluorescence microscopes, and can be run with different popular light sources. See the description below for more information. Cat no. 7001991-1 Request a quote or information

    Request a quote or order

    Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. We can also charge in $USD. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
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    StreptaClick® HRP

    Price range: 400 € through 1734 €
    StreptaClick® HRP Multiplex IHC Kit is an accelerated heat-treatment-free multiplex Tyramide Signal Amplification (TSA) staining solution with imaging for up to six colors for both frozen and FFPE samples in one day. This comprehensive kit is designed for researchers who demand ease of use, flexibility, precision and reliability in immunohistochemistry (IHC). The kit includes everything needed to label biotinylated antibodies with horseradish peroxidase (HRP) enzyme in solution utilizing our modified streptavidin StreptaClick®. As StreptaClick® removes the issue of antibody aggregation through monovalent biotin binding it is particularly suited for labeling primary biotinylated antibodies. The kit also features a novel HRP block buffer that eliminates the need for antibody-stripping by heat-treatment such as boiling between cycles. This feature maintains tissue morphology over multiple staining cycles making it ideal for both frozen and FFPE tissue sections, and higher degrees of multiplexing than comparable solutions. In combination, these features makes the protocol automation friendly and results for six color + DAPI experiments are achievable in one day.
    Order or request further information

    Request a quote or order

    Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

    Webshop

    Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® HRP Kit:
    Select options This product has multiple variants. The options may be chosen on the product page
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    StreptaClick® Color

    Price range: 339 € through 785 €
    StreptaClick® Color is a modified streptavidin that labels biotinylated antibodies with fluorochromes in solution, mimicking directly conjugated antibodies as we have removed the issue of antibody aggregation through monovalent binding. StreptaClick® Color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The labeling reaction is highly efficient and can be carried out at any volume, concentration, or buffer. Order or request further information

    Request a quote or order

    Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

    Webshop

    Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® Color Kit:
    Select options This product has multiple variants. The options may be chosen on the product page

    StreptaClick® Precision

    Price range: 400 € through 3694 €
    StreptaClick® Precision is a modified streptavidin designed for precise stoichiometric 1:1 conjugations. Currently, StreptaClick® Precision is available with exactly one azide or exactly one DBCO (dibenzylcyclooctyne). StreptaClick® Precision also features a unique cis-divalent design for enhanced monovalent binding to biotinylated proteins. In addition, the conjugation site and the biotin binding region are on opposite sides of StreptaClick® Precision, minimizing the risk of conjugates interfering with biotin binding. This design makes StreptaClick® Precision ideal for attaching bulky molecules without sterically hindering biotin binding and without causing aggregation when mixed with biotinylated antibodies in solution. Click-chemistry ready StreptaClick® Precision is currently available with a single azide or DBCO group for either copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. For oligonucleotides, we strongly recommend SPAAC click chemistry (DBCO/azide). Pre-conjugated, off-the-shelf StreptaClick® Precision based, pre-conjugated products are also available. These include StreptaClick® Precision with a single 14 mer DNA oligonucleotide or a single bright fluorochrome (488 nm or 647 nm). Custom conjugation as a service Please inquire to discuss the feasability of your project. Order or request further information  

    Request a quote or order

    Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

    Webshop

    Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® Precision Kit:
    Select options This product has multiple variants. The options may be chosen on the product page

    BiotinPure™ Antibody Biotinylation Kit

    503 €
    The BiotinPure™ Antibody Biotinylation Kit is a complete kit for easy biotinylation of antibodies at room temperature within 1 hour. The kit uses amine/ester chemistry to attach 2-3 biotins per antibody and includes a purification wash step that removes unreacted free biotin from the final biotinylated antibody solution. The antibodies to be biotinylated should be free from other proteins, such as BSA. The BiotinPure™ Antibody Biotinylation Kit is designed for use with StreptaClick® reagents. The biotinylated antibodies may also be used for other applications. Cat. no. 9000051-1 Order or request further information

    Request a quote or order

    Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally - a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

    Webshop

    Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your BiotinPure™ Kit:
    Select options This product has multiple variants. The options may be chosen on the product page

    Partners and collaborators

    Evident SLIDEVIEW™ VS200 Research Slide Scanner is offered with SpectraSplit® 7 for advanced multispectral scanning.

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    Nikon logo

    ECLIPSE Ti2-E inverted microscopes from Nikon are offered with SpectraSplit® 7 for advanced multispectral imaging.

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    ZEISS logo

    In partnership with Carl ZEISS we offer the StreptaClick® 8-Plex Multiplex IHC Kit for the ZEISS Axioscan 7 spatial biology system.

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    TissueFAXS CHROMA from TissueGnostics provides high-speed multispectral tissue imaging with SpectraSplit® 7 filter sets.

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    BioLegend logo

    BioLegend from Revvity offers more than 700 biotinylated primary antibodies of high quality, mainly for mouse and human targets.

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    Atlas Antibodies logo

    Atlas Antibodies offers antibodies for more than 12,000 Human Protein Atlas–validated targets, optimized for human IHC and/or ICC.

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    Synaptic Systems offers antibodies optimized for mouse and human FFPE sections. Many are available for purchase with biotin.

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